Escherichia coli replicates its genome through a finely tuned ensemble of proteins that assemble at the replication fork to form the replisome. Central to this machinery are the DNA polymerase III ...

Plasmids and the model bacterium Escherichia coli are at the heart of recombinant gene technologies. Plasmids are handled in test tubes with enzymes such as restriction endonucleases, ligases, and ...

We have gone further than ever before in creating life that is unlike anything that has evolved naturally. The genome of an Escherichia coli bacterium has been redesigned on a computer to use just 57 ...

Every time a cell divides, it must copy its DNA with extraordinary precision. But this process is constantly challenged by DNA damage. Among the most dangerous lesions are DNA interstrand crosslinks ...