The fluorescence-imaging technology in cells allows study the cellular mechanisms of the organelles with multiple imaging approaches. Different fluorescent proteins work as protein tags in detecting ...

Tunable Bessel beam two-photon fluorescence microscopy enables high-speed volumetric intravital imaging of subcellular dynamics within living mouse brains with fully tunable spatial resolution and ...

Super-resolution fluorescence microscopy has transformed our ability to visualise biological structures at the nanoscale by breaking the classical diffraction barrier of light. Traditional optical ...

Researchers at Peking University have developed DUET, a breakthrough dual-color miniature two-photon microscope that ...

Confocal microscopy is a specialized fluorescence imaging technique that scientists use to acquire images at greater resolution than conventional microscopy. 1 In addition to scanning the lateral x ...

FLIM principles. Schematic overview of fluorescence lifetime data acquisition and analysis in time−domain (td) and frequency−domain (fd) modes. In tdFLIM, photon arrival times are recorded after each ...

A new study presents a fluorescence imaging-based technique to measure the activity rates of individual scramblase proteins.

Gene expression studies can monitor biological processes using a “molecular beacon” (single-stranded DNA, ssDNA), a hairpin-shaped oligonucleotide containing a fluorophore (donor) and a quencher ...