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When setting up a Sanger sequencing reaction, each reaction should include template DNA, nucleotides, dideoxynucleotides, buffer, DNA polymerase, and ./Semasa membuat reaksi penjujukan Sanger, setiap tindak balas harus merangkumi DNA templat, nukleotida, dideoxynucleotides, buffer, DNA polymerase, dan .1 pointForward AND reverse primersForward OR reverse primersForward AND reverse probesForward OR reverse probes
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SOLVED: Using the "old" method of Sanger sequencing, the results were the following pattern of bands from agarose gel electrophoresis. The letters at the bottom of the lanes represent the ddNTP used in the reaction tube for that lane. What was the sequence of the DNA template used in the reaction? Write the sequence 5' to 3' using all caps, no spaces.
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